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The mixture was boiled, cooled to ∼60°C, and 0.5 g nipagin and 1 g nipasol were added, both dissolved in ethanol.
Cells were treated with 1 μ m 27-OH or with 1 μ m 24-OH (Steraloids, Newport, RI, USA), both dissolved in ethanol.
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α-TOS and VK3 (menadione) (both from Sigma) were dissolved in ethanol and DMSO, respectively, diluted in complete medium to the final concentration and added to cells at 0.1% of solvent (v/v).
EPA was first dissolved in ethanol or added directly to the complete media.
The drug brefeldin A (Sigma) was dissolved in ethanol and used at final concentration of 2 μg mL−1.
Melatonin (Sigma-Aldrich Chemical Co. St. Louis, MO, USA). was dissolved in ethanol and diluted with normal saline while in a darkroom.
Aluminum acetylacetonate of 0.001 to 0.005 M concentrations dissolved in ethanol was used as the precursor.
All tested samples were dissolved in ethanol.
GMO, F127, and TET were dissolved in ethanol.
They were then dissolved in ethanol for further use.
Pure PVP was dissolved in ethanol at 5 wt % concentration.
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