Sentence examples for both directions using an from inspiring English sources
The selected plasmids were subsequently sequenced in both directions using an ABI BigDye3.1 Terminator Cycle Sequencing Kit (Applied Biosystems, USA) with the ABI PRISM® 3700 DNA Automated Sequencer.
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Plasmid DNA from randomly selected clones was purified and sequenced in both directions using an ABI 3730XL DNA Analyzer.
Cycle sequencing reactions were carried out using the version 3.0 of the BigDye terminator chemistry (Applied Biosystems), using the primers listed in Table 4. Sequencing was performed in both directions using an ABI PRISM 3100 Genetic Analyzer (Applied Biosystems), and every nucleotide position was sequenced at least once from each strand.
PCR products of the expected size were purified by a PCR purification kit (Qiagen, Valencia, CA, USA) and directly sequenced in both directions using an ABI 3130 genetic analyzer with an ABI BigDye terminator v1.1 cycle sequencing kit (Applied Biosystems, Foster City, CA, USA).
PCR products were sequenced in both directions using an ABI PRISM 3730 DNA Sequencer.
Nuclear amplified fragments of three individuals for each population were sequenced in both directions using a modification of the Sanger dideoxy method as implemented in a double stranded DNA cycle sequencing system with fluorescent dyes.
Sequencing was performed in both directions using a PE Applied Biosystemsms 377 automated sequencer, using the Dyenamic Direct Cycle Sequencing Kit and the Dyenamic Energy Transfer Dye Primer Set from Amersham Int.
PCR products were purified using a MinElute96 UF PCR Purification Kit (Qiagen) and sequenced in both directions using a BigDye Terminator Sequencing Kit v.3.1 (Applied Biosystems) on a DNA multi-capillary sequencer (Model 3130XL genetic analyser; Applied Biosystems).
