Sentence examples for both directions using a from inspiring English sources

Nuclear amplified fragments of three individuals for each population were sequenced in both directions using a modification of the Sanger dideoxy method as implemented in a double stranded DNA cycle sequencing system with fluorescent dyes.

This DNA was sequences in both directions using a Beckman Coulter CEQ8000 following the manufacturer's protocol.

Long PCR products were sequenced in both directions using a primer walking strategy with a total of 306 primers.

PCR products were purified using a MinElute96 UF PCR Purification Kit (Qiagen) and sequenced in both directions using a BigDye Terminator Sequencing Kit v.3.1 (Applied Biosystems) on a DNA multi-capillary sequencer (Model 3130XL genetic analyser; Applied Biosystems).

Cycle sequencing reactions for each of the cloned PCR products were performed in both directions using a "primer walking" strategy, and the sequences of overlapping fragments were double-checked and assembled to obtain the complete sequence of each mitochondrial genome.

To determine the sequence of the CD20, we performed cycle sequencing on a 3130 DNA analyzer (Applied Biosystems, Foster City, CA, USA), directly from PCR-purified products, in both directions using a BigDye Terminator Cycle Sequencing Kit v3.1 (Applied Biosystems).

The selected plasmids were subsequently sequenced in both directions using an ABI BigDye3.1 Terminator Cycle Sequencing Kit (Applied Biosystems, USA) with the ABI PRISM® 3700 DNA Automated Sequencer.

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Plasmid DNA from randomly selected clones was purified and sequenced in both directions using an ABI 3730XL DNA Analyzer.

PCR products of the expected size were purified by a PCR purification kit (Qiagen, Valencia, CA, USA) and directly sequenced in both directions using an ABI 3130 genetic analyzer with an ABI BigDye terminator v1.1 cycle sequencing kit (Applied Biosystems, Foster City, CA, USA).

Cycle sequencing reactions were carried out using the version 3.0 of the BigDye terminator chemistry (Applied Biosystems), using the primers listed in Table 4. Sequencing was performed in both directions using an ABI PRISM 3100 Genetic Analyzer (Applied Biosystems), and every nucleotide position was sequenced at least once from each strand.