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Notably, ERα and JMJD2B were both detected at the ERα binding site in the JMJD2B locus, suggesting that JMJD2B regulates itself at the transcriptional level in concert with ERα, which is consistent with the previous genome wide analysis of ERα binding site [37].
Evc and Evc2 are both detected at the base of primary cilia in wild-type MEFs.
For example, miR_7 was detected at 5 dpf; miR_8 was detected at 72 hpf, 96 hpf and 5 dpf; miR_9 and miR_10 were both detected at 72 hpf and 96 hpf; miR_16 was detected at 72 hpf and 5 dpf.
The two transcript isoforms (a and b) of TRAES_2BS_CDE410A7D were both detected at S1, with only one isoform found at S2 and S3 and no isoform expressed at S4 (Additional file 9).
No Xenopus orthologue of Nanog has yet been identified, and although the homeobox transcription factors Ventx1/2 have been shown to function in a Nanog-like manner (Scerbo et al., 2012), none of these is detected as maternal transcripts, and all are strongly activated at MBT. Sox1 and Sox2 were both detected at low levels maternally before being strongly activated at MBT (Fig. 1D).
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In contrast to Notch1, N2/N1-GAL4 and the S1-resistant N2/N1-GAL4-LO protein are both readily detected at the cell surface by both flow cytometry and immunofluorescence (Fig. 6E H).
NO and the pro-inflammatory cytokine TNF- α have both been detected at elevated levels in osteoarthritic cartilage and both been implicated as possible contributors to chondrocyte death and cartilage damage.
Furthermore, while neither MX2 protein nor RNA was detected in prostate tumors, or indeed in normal prostate cells in the absence of cancer, both are detected at high levels in 'normal' stromal cells adjacent to tumors.
Both were detected at 6 weeks follow-up.
However, both were detected at the picomolar level, so regardless of the system complexity, LOD and LOQ are very satisfactory for both cases.
Moreover, one of our key finding was that LOH at marker D6S1581 in a combined analysis of both fractions, detected at primary diagnosis, was predictive for reduced OS.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com