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The observations in both data sets were related to thermoneutral conditions.
This analysis showed that both data sets were structurally highly similar, and only few compounds in the validation set were structurally different from the training set.
However, when both data sets were combined, then the conclusion of the European survey was further corroborated by the results of the Danish monitoring.
Therefore, to maximize reliability, both data sets were used in further analyses.
In the succeeding analyses, frequencies of mutants within both data sets were compared.
For some of the genes (e.g. Hsbp8) the database included 2 probe sets; in those cases both data sets were used in this meta-analysis.
Due to the low fungal frequencies both data sets were disconnected (many samples did not share any fungal species) and 22 samples in the sequence data were discarded.
A major concern of this biological-environmental comparison was that both data sets were collected at different spatial resolution, especially with satellite-derived data that were obtained on a much coarser scale.
Both data sets were generated using Agilent spotted oligonucleotide microarrays.
Also, variants present in both data sets were filtered out.
Both data sets were plotted as percent of control measurements.
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