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Both cultures were grown to an OD620 of 0.6.
Both cultures were grown at 37°C under agitation.
Both cultures were grown for 6 h before cells were collected using centrifugation.
Both cultures were grown to a final cell density OD600 of 0.1.
Both cultures were grown overnight at 37°C with shaking and subcultivated by 50-fold dilution in the respective growth media.
1-Octanol was added to one of the portions to a final concentration of 0.05% and both cultures were grown for 2 days at 37°C with shaking.
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Both axenic cultures were grown in plastic 150 ml flasks filled with artificial sea water medium, made by dissolving "Tropic marine" salt (Wartenberg, Germany) at 35 units of practical salinity.
Both liquid and plate cultures were grown under microaerophilic conditions (5% O2, 10% CO2, and 85% N2) generated with an Anoxomat gas evacuation and replacement system (Spiral Biotech) in gas evacuation jars.
However, in this study both test and control cultures were grown in medium supplemented with glucose.
Cultures were grown both with and without antibiotics to reduce the number of bacteria: 500 μg/ml penicillin G, 200 μg/ml ampicillin, 50 μg/ml streptomycin sulphate, and 50 μg/ml neomycin, modified from [ 19].
To validate our in silico data and test whether autophagy could be induced by nutrient limitation in P. falciparum, parasite cultures were grown in both serum-free medium and minimal medium devoid of the essential amino acid L-glutamine and serum, with minimal glucose.
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