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Genomic DNA was prepared from both control and experiment and a PCR was performed to amplify the signature tags (primers P1 and P2 (Table 4; 35 cycles; 20 s, 58°C; 20 s, 72°C; 30 s, 94°C; 10 s ramp between each step).
Absorbance at 475 nm for both control and experiment reaction fractions were recorded for each 5 min interval for 20 min.
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The absence of a nogo-LRP in the nogo/color conditions of both control and main experiment might indicate a failure to form a connection between the sensorimotor button pressing task and the linguistic processes required for speaking.
Narrowing this region is based on a simple statement that the measured genes are transcribed in both control and the experiment (knockout or heat shock).
In order to keep the flies stress free, three male and three female Drosophila flies were kept in each food vial in case of both control and treated experiments.
The data obtained from exogenous lactate infusion provide calculation of basal lactate production that were similar in both control and endotoxin experiments and were comparable to values reported in the literature with bolus lactate administration [ 35, 36].
Moreover, because it uses the same small-molecule affinity matrix in both the control and experiment samples, it circumvents the challenge of selecting an appropriate control matrix.
During the experiment, both control and exposed to prometryne crayfish showed normal feeding behavior.
At the end of the experiments both control and treated animals were killed and mammary glands fixed in formalin (right 4th gland), prepared for whole mounts (left 4th gland), or frozen for Western blot studies.
The animal glucometric and gravimetric characteristics measured at the termination of experiment, for both control and diabetic groups, are presented in Table 1.
Stomatal conductance was reduced by salinity in both control and Alethea-treated plants in our experiments.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com