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The participation rate for both control and cases subjects was 100%, so there was no selection bias.
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The distribution of all variants in both control and case groups is displayed in Figure 1.
ER-αand ER-βtranscripts occurred in both control and CAsE-PE cells.
Both control and CAsE-PE cells expressed only the type 1 isoform of 5α-R in the present study.
Both control and CAsE-PE cells exhibited optimal growth stimulation by E2 at a concentration of 1 μM.
As shown in Figure 2, AR protein in both control and CAsE-PE cells was expressed at the same level.
When DHT was added to cells growing in reduced steroid medium, both control and CAsE-PE cells exhibited growth stimulation.
ER-αand ER-βproteins were expressed in both control and CAsE-PE cells and were consistent with the data on mRNA.
In fact, AR was expressed at essentially the same level in both control and CAsE-PE cells (Benbrahim-Tallaa et al. 2005b).
Although both control and CAsE-PE cells showed similar levels of androgen receptor (AR), androgens were less effective in stimulating cell proliferation and AR-related gene expression in CAsE-PE cells.
Although hypomethylation of the ras gene can lead to activation, the K -ras promoter region, including the major transcriptional initiation site, was essentially unmethylated in both control and CAsE-PE cells (Benbrahim-Tallaa et al. 2005a).
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