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When both constructs were tested using the retinal explant assay, the −77/+57 human NXNL1 construct was found to be inactive as observed earlier (Figures. 3A and 4).
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Thus both fixation constructs were tested by alternating cycles of lateral compression/distraction (i.e. following the cylinder axis) and torque forces (i.e. counter-rotation of the cylinders about the central axis) using a universal testing machine (ElectroPulse E10000, Instron, High Wycombe, UK).
Since, all constructs were tested as both GAL4 DNA-BD as well as GAL4 AD fusions the 25 recorded associations actually corresponded to 12 unique effector domain interactions (see Table).
The resulting constructs were tested in both transgenic (Tg) zebrafish and mice.
All RNAi constructs were tested in 3T3 cells, CAD cells and primary neurons by both western blot and immunocytochemical staining.
Several retroviral constructs were tested for expression and coexpresssion of two genes in retinal cell types.
Methods: Four groups of suture anchor constructs were tested.
All shRNA constructs were tested for gene-silencing activity in MCF10A MycER cells either by quantitative PCR or western blotting analysis prior 3D culture screens.
Yeast cells carrying the 17 unique constructs were tested for β-carotene production using high-performance liquid chromatography (HPLC) (Fig. 2 and Supplementary Fig. 7a).
Full-length VDR constructs were tested in transient transactivation assays with 1α, 25-dihydroxyvitamin D3 (1, 25D3) (EMD Millipore, Billerica, MA) as the positive control.
The constructs were tested in two cell lines, CHO and HeLa.
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