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Both constructs were sequenced and the correct sequence obtained.
Both constructs were sequenced to confirm the presence of novel variation.
Both constructs were sequenced to confirm the absence of PCR-induced errors.
Two constructs of wild-type McbR (McbR1 221 (full-length), residues 1 221; McbR10 221, residues 10 221) were subcloned into the pRP1B bacterial expression vector, which contains an N-terminal His6-tag and Tobacco Etch Virus (TEV) cleavage site; both constructs were sequenced prior to subsequent experiments.
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Both packaging constructs were sequenced to confirm the absence of accessory genes in Δvif Δvpr Δvpu Δnef and the presence of complete open reading frames of vif, vpr, vpu, and nef.
The expression constructs were sequenced at both ends and selected constructs with correct sequences were named pPAMoz9 (Bm86), pPADec8 (Bd86) and pBaI (Ba86) and used for transformation of P. pastoris.
All the DNA constructs were sequenced by GENEWIZ (Suzhou, China).
All constructs were sequenced.
All constructs were sequenced to confirm identities.
All constructs were sequenced prior Leishmania transfection.
All constructs were sequenced (Dana Farber DNA Resource Core, Boston MA).
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