Sentence examples for both constructs were selected from inspiring English sources
Colonies coexpressing both constructs were selected by their ability to grow on selective medium (dropout without tryptophan and leucine).
For the F2 generation, progeny of 28 selfed F1 plants containing both constructs were selected in the same way.
Transformed cells containing both vector constructs were selected with nutrition-deficient medium plates lacking the essential amino acids, leucine and tyrosine.
Utilizing an internal PvuII restriction enzyme site orientation of promoter was checked and both sense and antisense constructs were selected for further experiments.
Immortalized MEFs Esco2fl/ Δ transfected with the constructs were selected for stable integration using the neomycin selection and FACS sorting.
To establish the different 293T-REx lines, we first generated a clone harboring an inducible shRNA transgene against SCML2 (" shL2" clone); then, we transfected the different transgenic constructs (N3, N3 SCML2AWT and N3 SCML2AΔRBR) into the shL2 clone and new clones harboring both the inducible shRNA and the inducible SCML2 constructs were selected.
Transformants containing both tio-3 and MSP promoter-TIO T-DNA constructs were selected on 15 cm MS agar plates supplemented with 10 mg/l ppt, 50 mg/l kanamycin, and 200 mg/l cefotaxime.
THY.AP4 cells expressing CubPLV bait constructs were selected on SC medium for leucine prototrophy, whereas THY.AP5 cells expressing NubG-prey constructs were selected for tryptophan prototrophy.
CL1-0 cellstablyly expressing constructs were selected with 800 µg/ml G418 (Calbiochem).
The construct was electroporated into Pichia pastoris, strain X33 and integrated constructs were selected on Zeocin-yeast extract-peptone-glucose agar plates.
Cells stably transfected with the ZAS3 constructs were selected with G418 (0.4 mg/ml) and incorporation of the constructs was validated by PCR of genomic DNA.
