Sentence examples for both constructs were linearized from inspiring English sources

Previous to transfection both constructs were linearized with HindIII and SacI restriction enzymes and purified from agarose gels by electroelution.

Both constructs were linearized with EcoRI to confirm insertion.

Constructs were linearized with ClaI and electroporated into R1 ES cells [20].

Constructs were linearized with Not1 and Ambion Kit Sp6 was used to make mRNA.

After DNA amplification, constructs were linearized and mRNAs synthesized using the T7 mMessage mMachine Kit (Ambion).

Targeting constructs were linearized with NotI and electroporated into 20 million cells (500 V, 25 µF; BioRad GenePulser II).

The pCAG-EGFP/RFP-miRNAint constructs were linearized by digestion with the restriction enzymes SspI and PciI.

The myoD and scl constructs were linearized with SalI and transcribed with T7 to generate antisense probes.

The constructs were linearized with the appropriate restriction enzyme (New England Biolabs) and cRNA was synthesized using in vitro run-off transcription with SP6 or T7 polymerase (Promega).

The RNAi constructs were linearized by NotI digestion so that they could be integrated into the rDNA spacer region of the T. brucei chromosomes.

For in vitro RNA transcription, channel constructs were linearized, transcribed (mMessage Machine T7 transcription kit, Ambion), and resuspended at a final concentration of 1 µg/µl.