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Cells expressing both constructs were identified on the basis of CyPet and YPet fluorescence intensity.
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A total of 21 constructs were identified.
Principal classes of constructs were identified and utilized to interpret what factors promoted the use of PA technologies by farmers.
Fourteen constructs were identified and grouped in three domains including perceived physical, social, or safety related characteristics of the neighborhood.
These constructs were identified as being particularly relevant for students in Newtown and arose from an earlier survey, conducted by the LA, into teachers' perspectives on attitudinal concerns.
Six concatemer constructs were identified and discarded (data not shown).
Cells expressing microinjected constructs were identified using GFP-expression.
Candidate constructs were identified by colony PCR, restriction digestion mapping and further confirmation by sequencing.
Transfected cells expressing dynamin or Eps15 constructs were identified by the GFP fusion tag.
The correct constructs were identified by PCR and restriction digests, and the plasmids transformed in BL21 cells.
The correctly ligated promoter constructs were identified and gel purified, quantitated by spectrophotometry, and confirmed by restriction enzyme digestion prior to transfection.
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