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Immunoblotting experiments showed that both constructs were expressed in T. brucei procyclic forms (Fig. 7A, B, upper panels) and incorporated [3H]Etn (Fig. 7A, B, lower panels).
Both constructs were expressed in Cos7 cells, incubated with equivalent amounts of GST-tagged importin α3 or β1Δ(1 462) immobilized on glutathione beads, and then incubated with 80 µM RanQ69L-GTP.
In cells transfected with both wild type and MΔ5 constructs with or without IBV infection, detection of similar amounts of M protein was observed (Fig. 5b, top two panels, lanes 1 and 2), suggesting that both constructs were expressed at similar efficiencies.
Both constructs were expressed, purified, and compared with one another.
3 hr after electroporation, the embryos were screened based on fluorescence to ensure that both constructs were expressed and that the correct region was targeted for each construct.
As expected, both constructs were expressed at a similar extent during early embryogenesis, as indicated by roughly comparable green fluorescence of embryos observed at the early blastula stage.
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Both constructs are expressed from their endogenous promoters.
This was not due to differences in protein expression levels as both populations were gated to have equivalent GFP signals, and western blotting showed that both talin head constructs were expressed at the expected molecular weights.
In addition an inverted-repeated construct was designed to inactivate DmMRG15 via the RNAi pathway, and RNAi constructs were expressed using both the Tet-on system and Geneswitch system.
Both the Art-Teth and WT N-ArtTeth constructs were expressed and efficiently transported to the cell surface in transiently transfected HeLa and HEK293-T cells (Fig. 5B,C).
hERG constructs were expressed in Xenopus oocytes using the pBluescript SK expression vector.
More suggestions(16)
both constructs were confirmed
both constructs were linearized
both constructs were tested
both constructs were selected
both constructs were modified
both constructs were modeled
both constructs were shown
both isoforms were expressed
both constructs were transfected
both groups were expressed
both constructs were identified
both constructs were grown
both mutants were expressed
both receptors were expressed
both constructs were verified
both enzymes were expressed
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