For genomic DNA, intron-exon boundaries were identified from conserved splice signals (GT/AG) and, where possible, by comparison with published cDNA sequences.
Although architecturally distinct, the catalytic triads of the bacterial and eukaryotic NHEJ polymerases are highly conserved and structurally superposable, suggesting possible convergent evolution leading to similar catalytic mechanisms.
This central metabolic pathway is highly conserved and ancient; it is therefore possible to compare enzymes from phylogenetically distant organisms [ 27].
In fact, even coding sequences are poorly conserved, and alignment is usually only possible between sequences representing the DNA-binding domain or other functional domains.
Most ICDSs correspond to authentic mutations and can therefore be compared between strains, making it possible to explore conserved and unique mutation events.
This is because promoters and transcription factor-binding sites often have lengthy overall gapped motif structure, and with the hash-interpolated Markov models, it is possible to capture the conserved higher order sequence information in the zde sample space.
That means a radical change of attitude, both about what it is realistically possible to conserve, and how to go about conserving it.
Even though the originally predicted CHV secondary structure of the 5′ UTR showed occlusion of both miR-122 binding sites, in the revised NPHV 5′ UTR, the second miR-122 seed site was both open and completely conserved, implying a possible hepatotropism of NPHV as observed for HCV (Table 1).
The PCR primer-binding sites for the amplicon were based on published sequences of geographical variants and were as highly conserved as possible.
In contrast, the short chain fatty acid utilisation regulator ScfA was very poorly conserved, with possible orthologs in A. nidulans, Aspergillus fumigatus and Neurospora crassa[ 20].
