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All DBL-domains contain both conserved and polymorphic regions targeted by surface reactive antibodies, but the conserved regions are most prominent in DBL3x and DBL5ε [36].
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Examination of all nucleotides, both at conserved and polymorphic positions enables the direct identification of new alleles, which may not be possible with techniques such as SSP and SSO typing [26].
Subsequent alignments using the complete sequences (containing both conserved N- and polymorphic C-terminal regions) were done to confirm results obtained with only conserved N-termini.
Recently, based on the conserved and polymorphic structure of LMW-GS genes, we developed a LMW-GS gene marker system and a full-length gene cloning method [ 21, 22].
We considered miRNAs in both conserved and non-conserved families (conservation across human, mouse, rat, dog, and sometimes chicken).
The application of this algorithm to a number of complex arbitrary BMDs shows that the approach is effective for both conserved and non-conserved textures.
We reason that a coordinated, not independent, action of both conserved and non-conserved residues determine enzymatic activity and specificity.
Known miRNAs comprise both conserved and non-conserved miRNA species.
TargetScan was applied considering both conserved and non-conserved targets.
The sequences generated, both conserved and non-conserved, increased the total number of miRNA families in Populus by 67 families.
These studies, mainly in subspecies of japonica, also identified significant numbers of both conserved and non-conserved miRNAs.
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