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MPO epitopes recognized by human sera were both conformational and linear epitopes [ 27, 28].
The epitopes targeted by the polyclonal antibody are unknown, but most likely include both conformational and linear epitopes on multiple structural proteins.
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Comparing the antibody repertoires between both twins we observed identical recognition patterns of conformational and linear epitopes before B cell depletion.
Furthermore, the R334 residue yielded neutralization sensitivity by antibodies recognizing diverse conformational and linear epitopes of gp120, suggesting that neutralization phenotype was a consequence of global structural changes of the envelope protein rather than a specific site epitope.
Phage peptide inserts were analyzed for conformational and linear homology using computational analysis; some peptides mimicked various domains of the original HIV-C Env, such as conformational V3 loop epitopes and the conserved linear region of the gp120 C-terminus.
We dissected the humoral immune response of rhesus monkeys with broadly reactive nAb responses using phage display; recombinant phage peptide sequences were evaluated for conformational and linear homologies to gp160 using computational analysis [52].
This indicates that the produced antigens contained conformational and linear epitopes that could be recognized by infection-induced antibodies.
Most conjugate proteins undergo both conformational and stability changes on ligand removal.
Our nanoscale experiments demonstrate that, upon interaction with a hydrophobic surface, P. aeruginosa type IV pili exhibit unique mechanical properties, that is, constant force plateaus most likely resulting from force-induced conformational changes, and linear force peaks reflecting nanospring behaviors.
Both logistic and linear regressions were used.
Values of p<0.05 were considered significant for both Pearson and linear-by-linear χ2 test.
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