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The accurate mass spectrum and retention time for all compounds were used for both confirmation and quantification purposes, while peak areas of EICs were used for quantification.
The accurate masses of the molecular ions in observed protonated molecule (M+H)+ and deprotonated molecule (M H)− for positive and negative ionization modes respectively were used for both confirmation and quantification purposes.
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Confirmation and quantification of (bio chemical reactivity.
Three mass transition pairs (72/55, 72/54 and 72/44) were used for analyte confirmation and quantification.
Samples that tested negative were eliminated but those which remained positive and caution samples were preceded to the HPLC for confirmation and quantification.
Should proteomic differences be identified with our qualitative MS protocol, the next step would involve confirmation and quantification, perhaps with ELISA-based techniques.
Furthermore, 4,5-dimethyl-nonane was excluded, because we were not able to obtain a high-purity calibration standard, and, hence, could not perform identity confirmation and quantification.
This led to the next step of evaluation of BBB opening in stroke, its localization by contrast-enhanced (CE -MRI, CE -MRIation and quantificonfirmation andropriate quantification
In AKI, functional confirmation and quantification of the severity of injury would allow the physician to determine the immediate need for renal replacement therapy.
Confirmation and quantification of morphologic apoptosis was performed using a Tdt-mediated dUTP nick-end labelling (TUNEL) kit to directly detect DNA fragmentation in situ.
Internationally, attention has increasingly turned towards rapid, nucleic acid amplification based approaches for the confirmation and quantification of MAP bacilli in faecal samples [ 11, 12, 22- 28].
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