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Both compounds were tested as catalysts for phenylacetylene polymerization but showed no catalytic activity.
Both compounds were tested against a number of TRP channels as well as against a broad panel of other non-related receptors and channels.
However, because 3-D similarity score calculation is much more computationally expensive, the four 3-D similarity scores (i.e., (ST^{{ST{text -}opt}}), (ComboT^{{ST{text -}opt}}), (CT^{{CT{text -}opt}}), and (ComboT^{{CT{text -}opt}})) between two compounds were "pre-computed" if both compounds were tested to be non-inactive in at least one common bioassay.
To test efficacy of complexes 1 and 2 in C3 cells, both compounds were tested using the well-established AlamarBlue assay.
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For determining the cytotoxic effect, a concentration range of both compounds was tested (0 80 nM VRL/0 400 nM VFL, dissolved in 0.9% NaCl).
The compounds were tested both as inhibitors of buckwheat phenylalanine ammonia-lyase (in vitro) and as inhibitors of anthocyanin biosynthesis (in vivo).
To determine both selectivity and potency, the compounds were tested in a HeLa WCE assay and categorised as mild (<50% inhibition), moderate (50 75%) and strong inhibitors (>75% inhibition).
All 30 purchased compounds were tested in both biochemical assays in concentrations ranging from 100 to 1 μM.
Selected compounds were tested on both cultured catecholaminergic cells and an in vivo model of hypertension; in each case, the candidates showed substantial mimicry of native CST actions, with preserved or enhanced potency and specificity.
A total of 170 compounds were tested in both the hERG assay and an action potential assay (isolated Purkinje fibres or isolated Langendorff-perfused hearts).
In one such study, an exhaustive list of hundreds of different conditions and compounds were tested in both homozygous and heterozygous diploid strains (10) and much of this data is included in the ScreenTroll database.
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CEO of Professional Science Editing for Scientists @ prosciediting.com