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For determining the cytotoxic effect, a concentration range of both compounds was tested (0 80 nM VRL/0 400 nM VFL, dissolved in 0.9% NaCl).
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Both compounds were tested as catalysts for phenylacetylene polymerization but showed no catalytic activity.
Both compounds were tested against a number of TRP channels as well as against a broad panel of other non-related receptors and channels.
However, because 3-D similarity score calculation is much more computationally expensive, the four 3-D similarity scores (i.e., (ST^{{ST{text -}opt}}), (ComboT^{{ST{text -}opt}}), (CT^{{CT{text -}opt}}), and (ComboT^{{CT{text -}opt}})) between two compounds were "pre-computed" if both compounds were tested to be non-inactive in at least one common bioassay.
To test efficacy of complexes 1 and 2 in C3 cells, both compounds were tested using the well-established AlamarBlue assay.
The antioxidant activity of compounds was tested by various methods.
The compounds were tested both as inhibitors of buckwheat phenylalanine ammonia-lyase (in vitro) and as inhibitors of anthocyanin biosynthesis (in vivo).
To determine both selectivity and potency, the compounds were tested in a HeLa WCE assay and categorised as mild (<50% inhibition), moderate (50 75%) and strong inhibitors (>75% inhibition).
Now experimental compounds are tested against both freely floating and attached myeloma cells.
Selected compounds were tested on both cultured catecholaminergic cells and an in vivo model of hypertension; in each case, the candidates showed substantial mimicry of native CST actions, with preserved or enhanced potency and specificity.
All 30 purchased compounds were tested in both biochemical assays in concentrations ranging from 100 to 1 μM.
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