Although ectopic Bik expression led to the formation of both Bcl-xL/Bik and Bcl-2/Bik complexes, we observed a dissociation of Bcl-2/Bim and Bcl-xL/Bim complexes as shown in Figure 5D.
However, for both the G-DID-DD and DID-DD complexes, we observed molar masses most consistent with formation of tetrameric complexes, i.e. (DID-DD 4/(FH2-DAD)4. DID-DD 4/
By comparing the behavior of different complexes, we observed that they distribute differently into the nucleus.
For the complex, we observed two peaks for the carboxyl carbon of PMAA.
Therefore this XCPE2 DNA-protein complex we observed did not appear to be formed sequence-specifically.
DOI: http://dx.doi.org/10.7554/eLife.06744.017 In the presence of the crosslinked complex we observed a two-step sequence of events.
With regard to QC expression in the EWN complex, we observed remarkable differences between mouse and human brain.
Therefore, the recycling model cannot account for the synergistic activation of Arp2/3 complex we observe in our assays.
All these processes contribute to the complex picture we observed at the molecular level.
Contrasting a high variability of the components of protein complexes, we rarely observed a loss of a whole complex.
