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Consequently, both colonies were removed from statistical analysis.
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More than 8000 colonies were removed from water meter boxes during the 12-year study period.
In experiments where the ant colonies were removed, the trees died.
Overnight grown individual colonies were removed using a sterile, plastic loop.
Additional nubbins from each of the twenty colonies were removed for genotyping.
Differentiated colonies were removed daily through aspiration, and the medium was replaced on a daily basis.
Dissociated colonies were removed from plates using a cell scraper and transferred into six-well low-attachment plates (Corning).
F. tularensis colonies were removed intact using a punch and fixed in 4% paraformaldehyde with 0.2% glutaraldehyde in PBS overnight at 4°C.
Depending on the cell growth rate, the colonies were removed from the plate and trypsinised (0.025% trypsin, 0.037% EDTA Na2, 2% chicken serum) into small cell clumps after five to ten days.
To produce EBs, ES cell colonies were removed from feeder cells using a detaching solution, which consisted of 0.25% trypsin supplemented with 1 mg/ml collagenase IV, 1 mM CaCl2, and 20% knockout serum replacement (KSR; Invitrogen, Carlsbad, CA).
Wild-type or csgD mutant colonies were removed from T agar after 2 or 5 days growth and placed into 2 mL sterile vials containing 0.2 g of 0.1 mm Zirconia/Silica beads (BioSpec Products Inc., Bartlesville, OK, USA); two colonies were added to each vial.
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