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For probe sets mapping to two different UniGene clusters, both clusters were considered for GO annotation.
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The clusters were considered as present on both chromosomes of the metacentrics in the phylogenetic analyses.
Consequently, the selected sizes of clusters were considered optimal, as larger clusters require more CPU time.
Those samples locating in typical locations or centroids of environmental clusters were considered as representative samples.
The set of the seed conformers representing these clusters were considered the "basis shapes" for that volume.
Allele frequencies among clusters were considered to be independent to prevent overestimation of cluster number [38].
30 clusters were considered as adequate.
Clusters were considered significant for p values £0.05.
Secondary clusters were considered significant at P ≤ 0.01.
These clusters were considered as operational taxonomic units (OTUs).
Primary clusters were considered significant at P ≤ 0.05.
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