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We see a spike in expression of both clusters at day 2, which we attribute to stress after being transplanted.
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Although the AUC of the number of clusters over time reflects the proliferative kinetics, cell clusters at day 15 is taken to represent the EPC yield and the end of the culture protocol.
In PDL tails with a Ngn3 transcript level above 60% of duodenum, 39.4±3.2% of β cells were YFP+ in small versus 15.4±1.1% in large clusters, at day 35 post-PDL.
Accordingly, BMP4 also significantly decreased the size of the acini structures as evidenced by a 40% reduction in the total area covered by the cell clusters at day 14 (P < 0.05, Figure 2C).
Pc3 occurrence rate decreases with the decrease of L both at day and at nighttime.
The calculations suggested that proliferation of pre-existing β cells can account for 86% of the β cell expansion observed in PDL tail (Supplementary Figure S4A) and that small clusters present at day 7 of PDL can expand their β cell numbers nearly threefold in the second week of PDL to generate larger clusters (Supplementary Figure S4B).
No difference was observed in location of primary branches of the phrenic nerves, secondary branches, and location and size of AChR clusters at postnatal day 0 (P0) between controls and mutants (data not shown).
(C) Cluster eccentricity at day 7 in cells grown under control and activated conditions with TGF-β in different collagen concentrations (4 and 6 mg/mL).
Focal adhesion (left panel) gene clusters were enriched at day 5 and RHO GTPase (middle panel) and actin cytoskeleton-related gene clusters (right panel) at day 8 upon HSF2 silencing.
Differentiation was analyzed both at day 7 and at day 5.
The expression of genes in this cluster was minimal at day 7 but peaked at day 10 postfracture, suggesting that these enzymes might be particularly important for the processes involved in the bone formation that becomes visible by day 10.
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