Your English writing platform
Free sign upExact(1)
The other two positive clones probably contained the same gene, as for both clones by using the primers puoRh_fw and puoRh_rv a PCR product was seen on gel.
Similar(59)
Retière, C. et al. Generation of cytomegalovirus-specific human T-lymphocyte clones by using autologous B-lymphoblastoid cells with stable expression of pp65 or IE1 proteins: a tool to study the fine specificity of the antiviral response.
We have investigated the genetic origin of the MGMT in a number of such MGMT-positive (Mex+) clones by using human MGMT cDNA and anti-human MGMT antibodies as probes.
We generated Set2 − germline clones by using the Set2 FRT chromosome.
We sequenced the selected clones by using the M13 universal primer.
Additionally, we identified 75 dominantly expressed genes in testis among the full-sequenced clones by using a DNA microarray.
The strategy we employed was preparation of a series of deletion clones by using exonuclease III and Mung bean nuclease.
Surveillance of MRSA clones by using molecular typing can provide invaluable information in the epidemiological, microbiological and clinical fields.
In Fig. 1C, we have summarized the distribution of gene annotations for the BBRAF clones by using Arabidopsis TAIR9 annotations.
Recombinant plasmids were extracted from positive clones by using the E.Z.N.A.R. Plasmid Miniprep Kit (Omega, Norcross, GA, USA) and identified by EcoRI restriction digestion (Promega).
Approximately 20 μg BAC DNA was purified from a 100 ml culture of the isolated BAC clones by using the Qiagen Plasmid Midi Kit (Westburg, Leusden, The Netherlands).
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.
Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com