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This suggests that although both classes of sites experience frequent adaptive fixation, non-domain codons may experience more adaptive evolution than domain codons.
We found that BCL3, RAD21 (cohesin), and MAX, were most significantly associated with p65 recruitment at both classes of sites.
On the other hand, H3K27ac, which is associated with permissive chromatin, and H4K20me1/H3K79me2, which are associated with transcriptional elongation, had similar levels at both classes of sites.
As for the upstream region, although %U level in the USE is consistently higher than background in strong poly(A) sites, it is also higher in weak sites, suggesting again that the 5' bias occurs in both classes of sites.
These results are supported by the observation that four-fold degenerate sites often have more extreme base composition than any intergenic regions, although both classes of sites are traditionally assumed to be evolving neutrally to the same base composition (Muto and Osawa 1987; Hershberg and Petrov 2010; Raghavan et al. 2012).
To further examine our two-round pipeline performance, we compared sites called only by the two-round pipeline vs. those called using just a single round of BWA to map and estimated both error rate and diversity for both classes of sites.
Similar(54)
For example, in common with previous studies, no successful predictor was found for mitochondrial non-synonymous changes [ 5, 14, 30], but our nuclear results were similar for both classes of site (Tables 1, 2, 3).
Both classes of ultrastable sites tend to be near transcription start sites (TSS; P <0.001, t-test; accounting for the distribution of sites on the 450 K; [see Additional file 1: Figure S5]).
This underscores the functional relevance of both classes of HS sites characterized in CD4+ T cells, TE-derived and non TE-derived, with respect to CD4+ T cell specific expression.
Non-significant for mitochondrial rates, this predictor has an r 2 of around 50% for both classes of nuclear site (Table 1).
A genome-wide scan for the occurrence of both classes of Fkh domain recognition sites in association with binding sites for known cardiac TFs showed an enrichment of combinations containing the two Fkh motifs in putative enhancers found within the noncoding regions of genes having heart expression.
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