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"Class 2" daf-2 mutants exhibit dauer-like traits as adults, while "class 1" mutants do not, yet both classes of mutants have extended lifespan [ 75, 93].
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Both classes of mutant, Pol II LOF and sua7 alleles, alter TSS distributions in a similar fashion, suggesting exacerbated TSS defects might underlie observed synthetic genetic interactions (see below).
We therefore generated dfmr1 mmp1 double null mutants and similarly found the same mutual repression of both classes of null mutant phenotypes, which is consistent with the TIMP overexpression analyses.
Reverse transcription polymerase chain reaction experiments revealed that transcript cleavage at the poly(A) site was impaired in both classes of increased readthrough mutants.
A PA14ΔlasR mutant and two independent PA14ΔgacAΔlasR double mutants behaved similarly in the assay used here, as shown by the fact that both of these classes of mutants abolished the characteristic staining of stomatal guard cells after infection using the membrane impermeable dye Sytox Green®.
The ABCE model was formulated from the analysis of floral homeotic mutants with organ identity defects in two adjacent whorls of the flower, and similar classes of mutants were described in both Arabidopsis and Antirrhinum, suggesting that the regulation of organ identity was highly conserved in evolution [ 112– 112].
We obtained and characterized two distinct classes of mutants altering NTP misincorporation and transcription slippage both in vivo and in vitro.
Our evaluation of transcript levels of genes encoding polysaccharide biosynthesis and cell wall loosening enzymes determined significant transcript reduction for both classes of enzymes in Li 2 mutant elongating fibers.
HCT116 cells were transfected with three TP53 mutants, selected as representative of different classes of mutants (Figure 3a).
Therefore, in certain circumstances, restriction endonucleases are effective reagents for the selection of new classes of mutants.
The two classes of mutants may differ in kinetic properties or cleavage mechanism.
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