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Both chromogens were from Biocare Medical.
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Calcein AM, Dulbecco's modified Eagle's medium (DMEM), EpiLife medium, ethylenediamine tetraacetic acid (EDTA), fetal bovine serum (FBS), HEPES, non-essential amino acids, penicillin/streptomycin/L-glutamine solution, RPMI-1640 without L-glutamine, sodium bicarbonate, and tetramethyl benzidine chromogen (TMB) single solution chromogen were from Invitrogen (Carlsbad, CA).
The secondary antibody and substrate chromogen were provided from the EnVision® System kit (K4009, Dako).
The HPLC approach for determining creatinine concentration and separation of creatinine from interfering chromogens is shown in Figure 2A C.
Both were from Nebraska.
AEC substrate-chromogen was purchased from Dako and cocktail protease and phosphatase solutions for protein harvest from Roche (protease inhibitor cocktail tablets –complete mini- # 836153001), Sigma (phosphatase inhibitor cocktail 2 # P5726-5 ml) and Calbiochem (phosphatase inhibitor cocktail IV # 524628).
DAB substrate-chromogen was purchased from Dako (K3468, Burlington, ON, CA).
Appropriate secondary antibodies were from Dako and DAB chromogen was used for visualisation.
Biotinylated secondary antibodies and HRP-conjugated streptavidin were from Vector (UK) and the chromogen substrate DAB from Sigma (UK).
Both are from Zara.
(Both are from Oberlin).
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