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One technology that has arisen to meet this challenge is activity-based protein profiling (ABPP) that uses activity-based chemical probes to broadly assess the functional states of both characterized and uncharacterized enzymes.
Furthermore, precursor peptides from both characterized and uncharacterized families were analyzed to identify common motifs.
These encompass both characterized and uncharacterized proteins, and make good candidates for ovule development regulators.
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Given that the computational screen identified the three known targets of SXL as well as a novel target of SXL, characterization of Rm62 using molecular genetics should provide important new insights into the function and regulation of both previously characterized and uncharacterized genes, the mechanisms of action of SXL, and the basis for sexual dimorphism.
Many of the characterized and uncharacterized genes have orthologues in model organisms, which can be used to study biological function.
Based on the most recently available information of the zebrafish gene annotation, these gene sets were classified as characterized and uncharacterized genes (Table 1).
Not surprisingly, many poorly characterized and uncharacterized genes were found exclusively or predominantly in air data relative to sequence from previously examined outdoor environments.
The MCCP approach also enables the experimenter to reveal subtle relationships between characterized and uncharacterized proteins.
In a typical analysis, characterized and uncharacterized genes are clustered based on sequence similarity measures and phylogenetic relationships.
A total of 195,631 SNPs were discovered in gene regions, which included 153,787 and 41,844 SNPs in characterized and uncharacterized gene regions, respectively (Table 4 and 6).
After the removal of characterized and uncharacterized gene reads, the remaining Roche 454 reads consist of uncharacterized repeats and other uncharacterized sequences, including introns, promoters, pseudogenes, unknown genes, and single-copy sequences in intergenic spaces.
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