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Data from both channels were used for processing.
Only spots with an intensity of at least 1.5 times above the local background in both channels were used for subsequent analysis.
Intensity values of spots showing hybridization intensity two standard deviations above background intensity in both channels were used for spot counting and correlation analysis on technical replicates of A. burtoni genomic DNA.
Only features with foreground to background ratio higher than 1.5 and coefficient of variation (CV, ratio between standard deviation of the background and the median feature intensity) lower than 0.5 in both channels were used for further analysis.
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In order to increase reliability of data, the following filtering criteria were applied: only slides with ≥ 20,000 (out of > 27,000) spots with a median signal intensity ≥ 3 SD above background in both Cy3 and Cy5 channels and a mean intensity ≥ 400 relative fluorescent units in both Cy3 and Cy5 channels were used.
Serpentine channels were used for both the methanol and air flows.
To further facilitate visualization, the images were combined into the different layers of an RGB image: the green channel of the RGB image was used for the spatially transformed angiographic image, and the other channels were used for both parts of the OCT C-scan.
A total of 59 channels were used for the recordings.
Tiko distribution channels were used to distribute posters, baseball caps, tee-shirts and other promotional materials.
When four channels were used, cy5 was labeled with pink.
Two of the output channels were used to drive the AOD, one to trigger data acquisition, and the remaining channel was used to debug scanning protocols.
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