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In both cells types, the increase in cGMP produced by CNP was identical in the presence of M372049 (10 µM; Figure 3), a concentration of the NPR3 antagonist that significantly reversed the effects of CNP on cell growth.
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In both cell types, the SOS response was activated, and levels of proteins such as SodB and CysK, which can counter oxidative stress, were increased.
Scanning electron microscopy analysis showed for both cell types the presence of cells inside the porous structure of the foams.
In both cell types, the cell uptake of compounds 1 3 was much lower than that of quercetin, but comparable to that of the glycoside isoquercitrin.
In both cell types, the nuclei of hydrated cells contained osmiophilic and widely dispersed chromatin (n, Fig. 2B, D).
For both cell types, the Kd for T-1249 was much smaller (one order of magnitude), accounting for a higher preference for the cell membrane.
Indeed, in both cell types the expected band could be detected (Figure 1B), demonstrating endogenous expression of KCR1 in native cardiac cells.
Interestingly, in both cell types the relocation (positional shift) caused by the contraction was always opposite to the preferred migratory direction.
However, where genes are regulated in both cell types the BL31 data can predict features required for their mechanism of regulation in LCLs.
In both cell types, the change in cell orientation became significant in a dcEF of 2 V/cm (21±2%, Mean±standard deviation) and highly increased at 5 V/cm (52±1.6%, Mean±standard deviation) (Fig. 1I and J).
Our studies revealed that in both cell types, the mouse receptor was also expressed on the nuclear membrane (Fig. 3F, HEK 293 data not shown) like its hGnRH-RI counterpart (Fig. 1F).
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