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However, the mRNA content of FasL was strongly upregulated in both cells lines by oxaliplatin treatment, being these effects more pronounced in the resistant cell line (20-fold) than in the normal one (4-fold) (Figure 6B).
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Cytotoxicity was assessed, for both cell lines, by the AlamarBlue® assay (Invitrogen, Eugene, OR) according to the manufacturer instructions.
Both BBS mutations impaired leptin-mediated pSTAT3 activation in both cell lines by affecting either the trafficking or the quantities of leptin receptor in these cells.
The rescue effect was induced in both cell lines by the CM, which was abrogated if BAY-11-7082 waddedded to the CM.
Transport inhibition studies showed dose dependent inhibition of fructose transport in both cell lines by the newly synthesized 6-deoxy-6-fluoro-d-fructose (6FDF).
The presence of Mycoplasma contamination was tested in both cell lines by PCR using the primers 5′-ACTCCTACGGGAGGCAGCAGT-3′ and 5′-TGCACCATCTGTCACTCTGTTAACCTC-3′, which amplifies the spacer regions between the 16S and 23S ribosomal RNA genes.
CTCFL/BORIS was one methylated CTA that became significantly upregulated in both cell lines by both drugs.
Indeed, CREB silencing led to decreased E2F-1 protexpressionsinn in both cell lines by 60% (A375SM) and 80% (C8161-c9), thus confirming our cDNA microarray findings (Figure 4A).
After preliminary testing of CaSR expression in eUCM-MSCs of both cell lines by immunocytochemistry, the first purpose of this study was to determine whether CaSR activation, by means of increased Ca2+ or calcimimetic NPS R-467 addition, could stimulate growth/proliferation of eUCM-MSCs.
MT1X and MT1G were both heavily induced in both cell lines by HDACi treatment.
The sub-G1 population was markedly increased in both cell lines by either olaparib or IR.
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