The pDNA/PPC/CS complex showed lower gene expression in both tumor cells, compared with that of pDNA/PPC complex (Figure 3).
No consistent differences of phosphorylation levels of pAkt (S473) and pMAPK p44-422) or in total Akt and ERK1/2 protein levels were observed in both CHKA-silenced cells compared with controls (Supplementary Figure 3).
In addition, LIS1 silencing reduced capabilities of cell invasion both in HCCC-9810 and CCLP1 cells compared with control cells (P <0.05).
PEI-g-HNTs/pDNA complexes show much higher transfection efficiency towards both 293T and HeLa cells compared with PEI/pDNA complexes at the equivalent N/P ratio.
Furthermore, ASNS knockdown resulted in increased proliferation and colony formation for both MHCCLM3 and MHCC97H cells compared with control cells.
Transfection of si-LIS1 resulted in efficient depletion of LIS1in both HCCC-9810 and CCLP1 cells compared with cells transfected with negative control (NC).
An examination of the YY1 mRNA and protein levels showed that they are both reduced in HS578T cells compared with MCF7 cells.
RT-PCR results showed that S100P expression was upregulated and Maspin expression downregulated by decreased latexin expression in both C3 and C7 cells compared with control BGC823 cells.
In vitro study showed that Ang II induced further increases in VEGF production in both the platinum-resistant cells, compared with those of their parent cells.
