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Both cell types used in the present study express TF [ 15, 36], and a possible enhanced TF activity could therefore be responsible for the increased cell motility observed after downregulation of TFPI.
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We have compared the maturation of RSPs produced by both cell types using SDS-PAGE and silver staining of the gel (Figure 8C).
The percentage of traced cells was comparable between both cell types using both Cre lines.
hESCs synchronized in early G1 or late G1 were differentiated into both cell types using defined culture conditions as described previously (Cho et al., 2012).
In addition, we enriched phosphopetides from both cell types using a TiO2-based strategy and quantified their differences based on ion abundance [ 27, 36].
However, further experimentation showed that this was not the case since a reduction in autophagy in both cell types using shRNA to genes required for autophagosome formation actually sensitized to CQ-toxicity.
Therefore, we performed a batch correction for both cell types using the following ANOVA model before further analysis of the data: y i = μ + B i + e i where y i is the expression level of the ith microarray, μ is the overall mean, B i is the batch to which the ith individual belongs and e i is the residual error.
Both tumor cell types used in these assays express αvβ3 and β1 integrins and can bind to the RGD sequence (data not shown).
The fact that the mediators that induced endothelial barrier enhancement differed in the two cell types used led us to wonder whether this S1P-like activity is limited to malignant lung epithelial cells and by secretion, to their microenvironment23.
(b) Shotgun MS signals measured in cell types used as sources for IPs.
Interestingly, our results indicate that only 10 13% of cells tested exhibited measurable blue-light sensitive current (out of 774 cells in total patched from the 4 cell types used, Fig. 1E).
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