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In both cell types, the SOS response was activated, and levels of proteins such as SodB and CysK, which can counter oxidative stress, were increased.
Scanning electron microscopy analysis showed for both cell types the presence of cells inside the porous structure of the foams.
In both cell types, the cell uptake of compounds 1 3 was much lower than that of quercetin, but comparable to that of the glycoside isoquercitrin.
While sbPBS provoked a strong oxidative stress response in both cell types, the expression of E-selectin, VCAM-1 and ICAM-1, responsible for the adhesion of MM6 cells to HUVECs, was induced in the latter through a proinflammatory paracrine effect.
In both cell types, the nuclei of hydrated cells contained osmiophilic and widely dispersed chromatin (n, Fig. 2B, D).
Interestingly, in both cell types the relocation (positional shift) caused by the contraction was always opposite to the preferred migratory direction.
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In both cells types, the increase in cGMP produced by CNP was identical in the presence of M372049 (10 µM; Figure 3), a concentration of the NPR3 antagonist that significantly reversed the effects of CNP on cell growth.
Therefore TRPC6 Ca2+ (mis)signalling has important implications for both cell types of the GBF in the progression of FSGS.
Once in culture, Dox (2 μg/ml) was added to both cell types for the duration of the experiments.
The obtained results clearly reflect the behavior of both cell types in the presence of different fibrinogen concentrations.
The maturation of both cell types within the hydrogels was confirmed by cardiac-specific biomolecular markers, including alpha-sarcomeric actin, actinin, and connexin-43.
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