These results, from a large number of purified T cell and monocyte samples from the same individuals, identify only a small number of genes with transcriptomic profiles associated with aging in both cell types, supporting the idea that some age-related changes may be cell-type specific.
Therefore, these results in individual cell types support that variation in cell type composition is not a potential confounding factor in this DNA methylome analysis from whole blood-derived DNA.
In contrast, immunogold staining was observed in both HFH and Huh-7.5 (Fig. 9G 9J, black arrows), confirming that both epithelial cell types supported HCV Jc1 replication in vivo.
The polymer was cytocompatible for all tested cell types, supporting cell viability and proliferation.
Further investigation of hmt-1 and pcs-1 revealed that these genes are expressed in different cell types, supporting the notion that hmt-1 and pcs-1 operate in distinct detoxification pathways.
The expression of SOD1 in the cytoplasm of multiple cell types supports a critical role for this protein in maintaining homeostasis.
Our method successfully identifies many disease-associated cell types supported by previously published literature, as well as highlighting associations worthy of further investigation, including associations involving MS and preeclampsia.
In this way, for each KEGG pathway, we obtain a final set of clusters using the informativeness metric that describe the distinct repertoire of expression patterns between cell types supported by the genes that participate in that pathway.
Further expression analysis is required to confirm these results, but finding similarly high EST counts between similar cell types supports the benefit of continuing these comparative approaches in more depth using RNA sequencing techniques.
In agreement with the perforation argument [ 52, 99], expression of Oikopleura Pax2/5/8 as in amphioxus, in the gill openings and anus, which lack sensory cell types, supports the hypothesis of an ancient function for Pax2/5/8 in the adhesion and fusion of epithelial layers and in the promotion of epithelial perforations.
