Although an explanation for this differential TLR responsiveness between follicular and MZ-B cells is missing, it cannot be attributed to TLR9 expression because both cell types express TLR9 similarly [ 108].
The PsychENCODE resource contains ~79,000 brain-active enhancers, sets of Hi-C linkages, and topologically associating domains; single-cell expression profiles for many cell types; expression quantitative-trait loci (QTLs); and further QTLs associated with chromatin, splicing, and cell-type proportions.
We investigate the predictions with respect to functional domains, cell types, expression levels, associated SNPs and chromosomal locations.
In all the cell types, expression with CA-Akt1 significantly enhanced transendothelial migration.
While sbPBS provoked a strong oxidative stress response in both cell types, the expression of E-selectin, VCAM-1 and ICAM-1, responsible for the adhesion of MM6 cells to HUVECs, was induced in the latter through a proinflammatory paracrine effect.
On both cell types, the expression of CD15 and CD33 was documented.
In both cell types, NICD expression led to decreased levels of endogenous Snail protein but upregulation of p21, an established Notch target gene.
Similarly, cytokines induced expression of the chemokines CXCL10 and CCL2 in both cell types, but CCL2 expression was higher in α cells basally and following cytokine-exposure.
Therefore, our results indicate that single-nucleus transcriptome sequencing provides an effective means to profile cell type expression dynamics in previously inaccessible tissues.
Confocal images illustrate the regional distribution and cell type expression of POMC and NPY.
