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In both cell populations, this plateau was reached around day 14 when cells were cultured in medium containing dexamethasone.
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For this aim, both cell populations were cultured in RPMI 1640 media supplemented with 2nM L-glutamine, 100 U/µL/ml penicillin/streptomycin (Life technologies), and 10% FBS (BioWhitacker) in a 96 wells plate for 12 hours.
Thus, both cell populations, non-CSLCs and CSLCs, benefit from this cooperation.
The first uses the state-space model, performs well when both cell populations are present and can track time-varying parameters.
By enriching with the 10% strongest DsRed-fluorescent cells, the productivity of both cell populations could be successfully improved.
Both cell populations show a similar histogram distribution with a few cells firing at high frequency.
Both cell populations senesced after five transplant generations.
We verified the purity of both cell populations by immunostaining.
Here we show that both murine and human progenitor cell populations express this receptor subtype on transcriptional and protein levels and respond to its agonists by reduced growth factor-induced cell cycle progression that leads to decreased myeloid, erythroid and lymphoid colony formation.
The first significant observation is that the majority of large genomic aberrations are present in both the GP3 and GP4 cell populations of this prostate cancer, presenting strong evidence of common origin or clonality between these two Gleason patterns.
Unlike to leucine, 19 mM arginine stimulated insulin release in both β-cell populations and this response was significantly higher as compared to 5.5 mM glucose alone or 10 mM leucine alone (Fig. 5A).
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