Sentence examples for both cell lysates and from inspiring English sources

For Western Blot and Zymography, both cell lysates and conditioned media (secreted proteins) were collected after various periods of stimulation.

The lead derivative displays a phosphorylation-induced fluorescence change that allows the visualization of real-time PKC activity in both cell lysates and living cells.

Then, a bicinchoninic acid assay was run to examine protein content in both cell lysates and extracellular vesicle samples.

Despite these concerns, we attempted to assay biochemical activities in vitro using both cell lysates and purified proteins.

In separate experiments, cells were lysed immediately following the 2 hr infection period and serial dilutions of both cell lysates and supernatants were plated to determine bacterial numbers.

To investigate the growth factor expression profile of hMNPs, PCR was used to detect mRNA and western blots were used to analyze protein expression in both cell lysates and the supernatant.

Both cell lysate and culture medium were measured.

Fig. 1B shows that treatment with a mixture of endo-O-glycosidase and sialidase reduced the apparent molecular weight of bands by∼5 kDa in both cell lysate and conditioned medium samples.

The proangiogenic protein, EDN1, showed the most dramatic and reproducible change in protein levels in both cell lysate and cultured media.

Cells incubated with probe 2 showed similar ratios of ShhN* to total ShhN in both cell lysate and in cell media, showing that ShhN* is released by the signalling cell.

To determine whether the low cytoplasmic level of mutant IGFBP5 was the result of increased secretion of the IGFBP5 into the media, we collected both cell lysate and conditional media from the stable cell lines and performed western blot analysis.