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To identify gene markers involved in EMT from EP156T to EPT1 cells, we profiled the gene expression of both cell lines using the Agilent Human Whole Genome Oligo Microarray, which contained 44 k probes.
These results were verified further in both cell lines using siRNA to specifically target IGF-IR (Additional file 2, Figure S1).
The lowest level of detection of both cell lines using the EGFR and EphB4 markers was 5 cells per ml of peripheral blood.
Cell proliferation using mitochondrial tetrazolium salt (MTS) assay was performed on both cell lines using DDs treated with either NM (control) or ZA (0.5, 1, 3, 5 or 10 μM, and either chelated (EDTA or EGTA 0.001%) or non-chelated.
The general presence of a nucleosome at the center of repressed RTSSs was also confirmed for all chromatin marks in both cell lines using a lower resolution mapping (Methods).
Analysis of replicate RNA samples from different batches of both cell lines using a microtiter plate format of the minisequencing method evidenced for the biological authenticity of the allelic imbalance detected using minisequencing in the microarray format.
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Both cell lines used (OV-MZ-6, SKOV-3) proliferated similarly in 2D, but not in 3D.
In both cell lines used, PHB was found labeled with GlcNAz sugar suggesting that O-GlcNAc modification of PHB is not cell specific (Fig. 1D).
Interestingly, both cell lines used for this study, MOLT-4 [46], [46] and K562 [47] express furin, or a protease with similar specificity.
As recipients we used HEK293 and CHO-K1, both cell lines used in basic research and industrial biotechnology.
Kefir caused the downregulation of TGF-α and the upregulation of TGF-β1 in both cell lines used.
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