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Both cell lines used (OV-MZ-6, SKOV-3) proliferated similarly in 2D, but not in 3D.
In both cell lines used, PHB was found labeled with GlcNAz sugar suggesting that O-GlcNAc modification of PHB is not cell specific (Fig. 1D).
Interestingly, both cell lines used for this study, MOLT-4 [46], [46] and K562 [47] express furin, or a protease with similar specificity.
Kefir caused the downregulation of TGF-α and the upregulation of TGF-β1 in both cell lines used.
As recipients we used HEK293 and CHO-K1, both cell lines used in basic research and industrial biotechnology.
Following normalization, we used the concentration of 30 nM for all siRNAs in further experiments, as it resulted in 80% transfection efficiency in both cell lines used.
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To further study the biological significance of this intra-tumoral dichotomy, we purified RR cells and Reporter Unresponsive (RU) cells derived from both cell lines using a flow cytometric cell sorter, and these subsets were cultured separately.
To identify gene markers involved in EMT from EP156T to EPT1 cells, we profiled the gene expression of both cell lines using the Agilent Human Whole Genome Oligo Microarray, which contained 44 k probes.
These results were verified further in both cell lines using siRNA to specifically target IGF-IR (Additional file 2, Figure S1).
The lowest level of detection of both cell lines using the EGFR and EphB4 markers was 5 cells per ml of peripheral blood.
Cell proliferation using mitochondrial tetrazolium salt (MTS) assay was performed on both cell lines using DDs treated with either NM (control) or ZA (0.5, 1, 3, 5 or 10 μM, and either chelated (EDTA or EGTA 0.001%) or non-chelated.
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