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The cleavage of caspase-3 and PARP was also detected in both cell lines treated with adriamycin.
We then measured caspase activity in both cell lines treated with DT-GMCSF and found similar activation to that measured in U937 cells (Fig. 5B,E).
SEM clearly showed morphological cellular transformations in the direction of cellular death in both cell lines treated with EGFR inhibitors.
Colony-forming assays were performed on both cell lines treated with antisense or sense oligonucleotides and gemcitabine.
MTT assay was very low (<3%) in both cell lines treated with 0 0.5 mg L−1 Cd.
In the present study, both cell lines treated with kefir exhibited an increase in optical density indicating an increase in apoptosis.
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Our results show that E2F1 was significantly downregulated in both the cell lines treated with VPA, while the repressor E2Fs were not affected in either of the cells lines upon VPA treatment (Fig. 1c).
In contraposition, and again in both tumor cell lines treated with TNF-α or CIS, the phosphorylated fraction was drastically incremented (P < 0.001 vs CTL, PTX).
Similar to the above results, both the cell lines treated with CFX at 200 μg/ml and 400 μg/ml showed significant shift towards left.
Furthermore, as expected, characteristic "ladder" pattern of apoptosis was also observed in both the cell lines treated with either MFX (400 μg/ml) or CFX (200-400 μg/ml) Fig. 2c.
As shown in Figure 3b, immunoprecipitation of FLAG-CSDA followed by immunoblotting with pYB-1 of lysates from both stable cell lines treated with IM results in profound decrease in S134 phosphorylation of CSDA, revealing Bcr-Abl-dependent phosphorylation.
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