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Our results support a more effective action of CP 55-940 to produce cell death of both cell lines through apoptotic mechanisms.
The results of the comet assay suggest that LD induced DNA damage in both cell lines through the induction of double strand breaks, single strand breaks or alkali label sites.
Examination of genes regulated by HP-14 in 2102EP and 2102EP-R revealed that HP-14 affects proliferation of both cell lines through activation of different pathways.
Activation of the adenylyl cyclase by forskolin (100 μ M) and treatment with the cAMP analogue dbcAMP (1 m M) induced VASP phosphorylation in both cell lines through PKA.
Of 566 genes defined as MPA upregulated, about 64% were regulated through PR in T47Dco cells, about 21% through AR in Y-AR cells, and about 15%8888 genes) in both cell lines through both AR and PR.
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Time-lapse microscopy also confirmed that both cell lines migrated through spreading and not through proliferation (Additional file 4 and 5).
In both cell lines, CRH induced FasL through CRHR1.
Heparinase increases tumor invasion in both cell lines and spontaneous tumor models, through both extracellular matrix remodeling and increased peritumoral lymphangiogenesis [ 52].
As shown in Fig. 6C, ribosomes of both cell lines displayed the ability to pass through the premature stop codon although this dysfunctional property was 30% higher in the most aggressive cells MA- cells than the control cells MP.
This study show that CD40L expressed on adjacent non tumoral cells induce a multidrug resistance to apoptosis in breast carcinoma and NHL cell lines, through both caspase dependent and independent pathways in NHL cell lines, and through a caspase independent pathway in breast carcinoma cell lines.
Furthermore, we recently showed that the depletion of galectin-1 in various human glioma cell lines through both stable knockdown and transient targeted small-interfering RNA (siRNA) treatment induces an intracellular accumulation of integrin-β1 coincident with a diminution of integrin-β1 at points of cellular adhesion at the cell membrane, without altering the β1 gene expression level (28).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com