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Both cell lines show an increase in population doubling time in the MCLs (Fig. 4a, b).
On day 8 both drugs in both cell lines show tRNA synthetase downregulation.
Figure 6A shows that both cell lines show enhanced phosphorylation of p53 Ser15, the specific residue phosphorylated by ATM/ATR (albeit higher in the PyV MT/jnk2+/+ cells).
Both cell lines show an immediate decrease in respiration but no significant change in glycolysis before cells started to die, seen by a significant decrease of impedance at about 8 9 h in HCT-116 cells and at about 10 11 h in HT-29 cells (Fig. 2F, I).
Both cell lines show human karyology.
In addition, both cell lines show elevated SHP2 expression [ 28].
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The KO of PTPRS for both cell lines shows a dramatic increase in phospho-ERK and phospho-AKT.
Furthermore, both cell lines showed reduced expression of the cyclin-dependent kinase inhibitor p21, an important mediator of the cell cycle exit requisite for myogenic differentiation (Fig. 2d).
Analysis of BrdU/DNA cell cycle in both cell lines showed that M induced cell cycle perturbations in both BrdU positive and BrdU negative cells.
Comparison of serum-free conditioned medium from both cell lines showed conspicuous differences in the levels of several proteins, including a quartet of high molecular weight proteins (≥140 kDa).
We found that RR cells derived from both cell lines showed a significantly higher IC50 (inhibitory concentration at 50%) to doxorubicin than RU cells (GOTO, 2 μM versus 0.71 μM, p < 0.001; SK-N-SH, 1.2 μM versus 0.51 μM, p < 0.001) (Supplementary Figure 2A and 2B).
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