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Figure 3 shows an example of genes that fell into two biological processes (GO Terms) that were common to both cell lines, "response to organic substances" and "regulation of transcription".
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As Figures 7A-C and 8A-C show, acute as well as chronic exposure to nicotine significantly induced the protein expression of both metalloproteinases and EGR-1 in both cell lines, responses significantly reduced by simultaneous exposure to GABA.
Data regarding these two groups of cell lines response to afatinib were first established by determining each individual cell line's IC50.
The cell lines response to the chemical agents is quantified by the IC50, the drug concentration necessary to inhibit the growth of a exposed cell line culture to 50% relative to the untreated control.
Reviewer 1: Overall, this is an interesting study that uses multiple different types of profiling technologies to establish how multiple myeloma cell lines response to bortezomib, the common anti-multiple myeloma treatment.
Two dose-dependent patterns of MTS growth were observed in MTSs of both cell lines in response to IT or toxin treatment: (1) complete inhibition of MTS growth ('sterilisation'); and (2) partial/complete inhibition ('heterogeneous response').
Similar pathways were altered in both cell lines in response to hypoxia.
Arrest of the cells in the S phase of the cell cycle implies that DNA synthesis is being inhibited in both cell lines in response to BaP exposure.
Similar results were also found in both cell lines in response to blockage of ER stress by silencing of PERK (Fig. 5c).
We found that common pathways were significantly enriched in both cell lines in response to hypoxia, although not necessarily the same genes.
Although TP53 is known to be involved in both apoptosis and DNA damage repair, its expression was downregulated in both cell lines in response to UV treatment (Fig. 4a).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com