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This indicates common processes which occur during aging in both cell lines rather than cell line specific changes.
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Complicating the picture, many investigators use fetal fibroblast cell lines rather than primary cell cultures [46], [47], [48].
Another limitation of our study is the use of cell lines, rather than brain tissues, for expression analysis.
For this reason, we chose Vero and Vero E6 cell lines rather than any other human or non-human mammalian cell lines to produce VLPs.
We elected to use cell lines, rather than primary tumors, because these cells are readily available to any investigator who wishes to confirm or extend our genomic findings.
There were two reasons for producing stable cell lines rather than performing transient transfections with plasmids expressing shRNA-TIP5 or miRNA-TIP5 sequences.
Thus, the modulation of these proteins is unlikely to be associated with LBH589-mediated potentiation of TRAIL-induced apoptosis in these cell lines; rather, increase in Bcl-2 and Mcl-1 may counteract LBH589's effect in sensitizing pancreatic cancer cells to TRAIL-induced apoptosis.
We focused on analyzing normal tissues or primarily tissue derived cell lines, rather than differentiated cell lines or cancer genomes.
However such models rely on transplantation of clonal tumour cell lines rather than tumours being induced in situ by asbestos.
In addition, NUF2 mRNA was highly expressed in a set of human pancreatic cancer cell lines, rather than HEK-293 cell line.
A major difference compared with previous screens is the evaluation of heterogeneous panels of cell lines rather than engineered isogenic cell lines.
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