Sentence examples for both cell lines pretreated from inspiring English sources
The suppression of basal and H2O2-induced LIP was also further confirmed in both cell lines pretreated with the highly lipophilic strong iron chelator SIH for 18 h prior to H2O2 treatment (data not shown).
To sum up, our data demonstrate enhanced radiosensitivity in four solid tumour cell lines pretreated with NVP-AUY922 or NVP-BEP800.
RNA samples were prepared from cell lines pretreated for 5 days in RPMI 1640 medium supplemented with 10% bovine calf serum (R/BCS) and subsequently cultured for the indicated durations in R/BCS medium supplemented with oestrogen (E2), epidermal growth factor (EGF) or R/BCS medium without further additions.
(D) The levels of SCARB2 expression on the cell membrane from 293A-hSCARB2 celineine pretreated with or without PNGase F were monitored by flow cytometry assay.
(E) Binding affinity comparisons of EV71 to 293A-hSCARB2 celineine pretreated with or without PNGase F. (F) Infection efficiency of EV71 to 293A-hSCARB2 celineine pretreated with or without PNGase F. Results shown are the mean ± SEM of three independent experiments for panel (D F).
EV71-GFP was used to infect 293A-hSCARB2 celineine pretreated with or without PNGase F. Fluorescence of GFP was determined 16 h post infection and EV71 infectivity was calculated and normalized to the infectivity to 293A-hSCARB2 celineine without any treatments, which was considered as 100%.
When the cells were pretreated under hypoxia for 48 hrs and then cultivated under normoxia, MTT values of both cell lines were significantly higher than those cells always kept under normoxia (P < 0.05).
As shown by immunoassay, the levels of soluble uPAR upon lipid raft disruption in the conditioned medium were increased in cells pretreated with a lipid raft disrupting agent at the 24 hr time point in both cell lines.
In both cell lines, TNF-α induced NF-κB luciferase activity above background by ~2.5 fold in control cells, whereas in cells pretreated with AR-18 the levels of NF-κB luciferase remained lower than baseline, and were not significantly different from those seen with AR-18 alone (Fig. 2C).
