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Both cell lines present differences in their mode of proliferation, HepG2 cells in tridimensional space opposite to HuH7 cells which proliferate as monolayers and HepG2 cells which proliferate faster and their size is smaller than that of HuH7 cells (Table 2).
This higher vimentin expression in IIB-BR-G-MTS6 cells was also confirmed by confocal microcopy (p < 0.05) (Fig. 5B) which also showed that both cell lines present highly organized actin F structures and that vimentin is mainly localized at cell extremes in IIB-BR-G-MTS6 cells which displayed a spindle-like morphology (Fig. 5C).
This observation was expected since both cell lines present features of mesenchymal phenotype.
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Both cell lines presented a GlcNAc concentration-dependent change of O-glycosylation pattern (Supplementary Figure 6).
Both cell lines presented microvilli but those of IPEC-1 were less and smaller.
In addition, both cell lines presenting hypermethylation of hMLH1 had a similar IC50 showing consistency in our results.
Both prostate carcinoma cell lines present active autocrine stimulatory loops involving EGFR signalling, as is typical for de novo human prostate carcinomas (Wells, 2000); we minimise this effect in vitro by altering culture conditions, although this cannot be negated during the invasion studies (Xie et al, 1995).
In a subsequent study, two main cell lines present in the dermis and epidermis, namely keratinocytes and fibroblasts, were cultured on top of the ELR-click fibers.
These cell lines present different degrees of sensitivity to artemisinin.
The results from in vitro studies in cell lines present a huge challenge in their evaluation and extrapolation to human cancers absent the data to make such a leap.
Skin Fibroblasts from two non-AD controls (2 females, mean age 63.5±9.2 years) and two AD patients (1 female, 1 male, mean age 66.0±11.3 years) were selected from the cell lines present in our cell repository originally established in 1993 [33].
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