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While the crude extract of strain CG 22 had cytotoxic activity against both cell lines, only the most polar fraction (F6.1) was active against the MCF-7 cell line (IC50 of 45 μg mL−1) (Table 2, Fig. 3c).
From a total of 54 aberrations in both cell lines, only six strong aberrations could be found in common: losses at 1q, 8p, 10p and 13 and gains at 10q and 17q.
Our Western blot experiments to analyze the AMPK activation status in cells grown in different glutamine availability, showed that in correlation with a drop of ATP levels in both cell lines, only in normal cells, in which the ATP decrease was greater (see Figure 5I, L and M), a reliable and glutamine-dependent increase of AMPK activation was observed (Figure 8B, 1 mM and 0.5 mM glutamine samples).
Among all the detected metabolites common to both cell lines, only the concentrations of sarcosine and glycine change in opposite directions, i.e., in cells grown under hypoxic conditions.
In both cell lines only minimal caspase-3 cleavage could be detected, worth mentioning is the minor dose response detectable for the curve shift to the right within Cal-78 cells.
Although a gradual increase of the internalized fraction was observed for both cell lines, only 18.3 ± 0.8%% of the total cell-associated radioactivity were internalized at 24 h after incubation start for DU-145 and 16.2 ± 0.6 % for MCF-7 cells.
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Imatinib and doxorubicin lead to apoptosis induction in both cell lines, but only in MCF 7 cells a further increase in apoptosis can be detected when drugs are combined.
Either PTHrP or IL-8 stimulation led to increased secretion of MMP-13 in both cell lines but in a significant manner only in MDA-MB-231 cells.
In both AR-negative cell lines, only PC3 cells reactive AR expression after treatment with DZNep and SAHA.
When these four pairs of gRNA/Cas9 endonuclease system were introduced into both HPV16 positive and HPV16 negative cell lines, only HPV16 positive SiHa and Caski cell lines displayed high rate of apoptosis.
While both glycosides showed antiproliferative activity on cancer cell lines, only the α-anomer caused endoplasmic reticulum (ER) stress and cell death on C6 glioma cells.
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