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As shown in (Fig. 7B), and (Fig. S5B), the knocking-down of H19 RNA resulted in nearly complete attenuation of p57kip2 induction in response to hypoxic stress in both cell lines; moreover, using two different siRNAs targeting H19 strongly indicates the specificity of the results.
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Figure 5A and B demonstrates that there is a major loss of co-regulation among peroxisome-related genes in both HepG2 and Huh7 HCC cell lines; moreover, this loss mainly results from dysregulation of genes involved in peroxisomal fatty acid β-oxidation (e.g. ACOX, EHHADH, ACAA1) and genes involved in the control of the H202 metabolism (e.g. CAT and SOD).
However, DCA treatment failed to reduce Δ ψm in any tested MM cell lines, moreover, DCA at doses of 10 and 25 mℳ tended to increase the Δ ψm in RPMI8226-TGL cells.
However, we failed to identify differences in the ERBB4 intracellular form in CHLA-9 versus CHLA-10 cell lines; moreover, no differences in nuclear ERBB4 expression were observed following subcellular fractionation, even after treatment with the phorbol ester, PMA, known to activate TACE (Zhang et al, 2001) (data not shown).
Viability of both cell lines was drastically inhibited; moreover, the colony formation capacity was significantly reduced, demonstrating long-term effects even after removal of the drug.
Moreover, both cell lines displayed homozygosity for all tested SNPs (Supplementary information, Figure S3B and Table S3).
Moreover, in both cell lines, fumarate, malate, and succinate are not significantly different between cells grown in hypoxic versus nonhypoxic conditions.
Moreover, in both cell lines, cell-cycle alterations correlated with an increase in cyclin E expression, which typically reached a peak at the G1 S transition.
Moreover, when both cell lines were grown under hypoxic conditions, AU565 cells exhibited a much stronger hypoxia response as measured by higher mRNA levels of several hypoxia-inducible genes (Additional file 4, Figure S7).
Moreover, in both cell lines the effect of the drug on the Ras-MAPK pathway could be observed at a position downstream of Ras but upstream of MEK (i.e., at the level of Raf).
Moreover, in both cell lines the effect of different doses of RGZ (1, 10 and 20 μ M) on the expression of MMP-9 and of its specific tissue inhibitor TIMP-1 was investigated.
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